| Course Unit Code | 636-3027/02 |
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| Number of ECTS Credits Allocated | 5 ECTS credits |
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| Type of Course Unit * | Choice-compulsory type B |
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| Level of Course Unit * | Second Cycle |
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| Year of Study * | Second Year |
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| Semester when the Course Unit is delivered | Winter Semester |
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| Mode of Delivery | Face-to-face |
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| Language of Instruction | Czech |
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| Prerequisites and Co-Requisites | Course succeeds to compulsory courses of previous semester |
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| Name of Lecturer(s) | Personal ID | Name |
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| VOD37 | prof. Ing. Vlastimil Vodárek, CSc. |
| Summary |
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| The course is dealing with modern methods of structure characterization of materials which are exploited in materials and biomedical engineering. Students will learn about possibilities and limitations of individual experimental techniques, about methods of specimen preparation. Structure assessment of engineering and biological materials will be demonstrated on practical examples. |
| Learning Outcomes of the Course Unit |
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- explain principles of the most important microscopical techniques for characterization of engineering and biological materials,
- explain possibilities and limitations of individual techniques,
- learn, how to prepare specimens from engineering and biological materials,
- demonstrate applications of modern microscopical techniques on practical examples. |
| Course Contents |
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1. Basic goals of structure characterization of materials in biomedical and materials engineering.
2. Products of electron interaction with a specimen.
3. X-ray absorption in solids. Diffraction of electrons on crystal lattice in cristalline materials.
4. Microscopy techniques based on focused electron beam.
5. Transmission electron microscope (TEM) – principle, spatial resolution, amplitude and phase contrast, diffraction analysis in TEM.
6. Problems related to investigations on biological specimens in TEM. Low voltage TEM.
7. Analytical electron microscopy.
8. Preparation of specimens for TEM from engineering materials(extraction replicas, thin foils – electrolytic thinning, FIB).
9. Preparation of specimens for TEM from biological materials (chemical and physical methods).
10. Scanning electron microscope (SEM): principle, spatial resolution and depth of focus, images in secondary and backscatter electrons, diffraction analysis in SEM.
11. Scanning electron microscopes with deteriorated vacuum level in the specimen chamber – low vacuum SEM (LVSEM) and environmental SEM(ESEM), possibilities and limitations of their applications for non-conductive specimens.
12. Preparation of biological specimens for SEM (crygenic and chemical methods).
13. X-ray microanalysis in SEM – energy and wave length dispersive analyses, qualitative and quantitative analyses, limits of detection.
14. Typical applications of electron microscopy in materials and biomedical engineering.
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| Recommended or Required Reading |
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| Required Reading: |
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WILLIAMS, D. B. and C. B. CARTER. Transmission electron microscopy, A textbook for materials science. 2nd edition, Springer US, 2012.
ISBN 978-0-387-76502-0.
DAWES, C. J. : Introduction to Biological Electron Microscopy: Theory and Techniques, Ladd Research Industries, Inc. Publisher Burlington, Vermont 1988.
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VODÁREK, V. Strukturně fázová analýza, Ostrava: VŠB – TU Ostrava, 2013. Dostupné z:
http://katedry.fmmi.vsb.cz/Opory_FMMI/636/636-Strukturne_fazova_analyza.pdf.
KARLÍK, M. Úvod do transmisní elektronové mikroskopie, Praha: CVUT, 2011. ISBN 978-80-01-04729-3.
HULÍNSKÝ, V. a K. JUREK. Zkoumání látek elektronovým paprskem. Praha: SNTL, 1982.
DAWES, C. J. : Introduction to Biological Electron Microscopy: Theory and Techniques, Ladd Research Industries, Inc. Publisher Burlington, Vermont 1988.
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| Recommended Reading: |
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| GOLDSTEIN, J., et al. Scanning electron microscopy and X – ray microanalysis. 3rd edition, New York: Springer US, 2003. ISBN 978-0-306-47292-3. |
WILLIAMS, D. B. and C. B. CARTER. Transmission electron microscopy, A textbook for materials science. 2nd edition, Springer US, 2012.
ISBN 978-0-387-76502-0.
GOLDSTEIN, J., et al. Scanning electron microscopy and X – ray microanalysis. 3rd edition, New York: Springer US, 2003. ISBN
978-0-306-47292-3.
KALINA, T. a V. POKORNÝ: Základy elektronové mikroskopie pro biology. Praha: Univerzita Karlova, 1981. |
| Planned learning activities and teaching methods |
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| Lectures, Tutorials, Experimental work in labs |
| Assesment methods and criteria |
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| Task Title | Task Type | Maximum Number of Points
(Act. for Subtasks) | Minimum Number of Points for Task Passing |
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| Credit and Examination | Credit and Examination | 100 (100) | 51 |
| Credit | Credit | 30 | 15 |
| Examination | Examination | 70 | 36 |