Skip to main content
Skip header

Laboratory Practicum

* Exchange students do not have to consider this information when selecting suitable courses for an exchange stay.

Course Unit Code546-0844/02
Number of ECTS Credits Allocated4 ECTS credits
Type of Course Unit *Compulsory
Level of Course Unit *Second Cycle
Year of Study *First Year
Semester when the Course Unit is deliveredWinter Semester
Mode of DeliveryFace-to-face
Language of InstructionCzech
Prerequisites and Co-Requisites There are no prerequisites or co-requisites for this course unit
Name of Lecturer(s)Personal IDName
PER34doc. Mgr. Eva Pertile, Ph.D.
The aim of the course is to broaden and deepen the knowledge of chemical laboratory techniques, including the acquisition of practical skills focused on the analysis of both waste, raw materials and other types of environmental samples (air, water, PM, green matter, etc.). The aim of the course is to deepen students' readiness to solve analytic problems by selected instrumental methods not only in the laboratory but also in the field. Provide an overview of selected analytical methods and procedures used to analyze real environmental samples and chemometric evaluation of analytical results.
Learning Outcomes of the Course Unit
Knowledge rendered upon completion of the course: Obtaining increased knowledge and understanding of the reactivity of organic and inorganic compounds. Depending on the previous study it is to gain knowledge on sampling from different environments (eg.: surface water, soil, waste), and their further processing lab, including presentation and evaluation of the results.
Skills rendered upon completion of the course: After successfully completing the course will graduate a comprehensive overview and understanding of the theoretical and practical aspects in environmental fields.
Course Contents
1. Introduction, OSH, laboratory glassware + tools.
2. Basic laboratory work; calculations: composition of solutions, titration factor, volumetric factor, pH value, basic statistical parameters.
3. Sampling and analysis of waste sample: Sampling of waste and sample handling (preparation part, sampling and preparation of laboratory sample, sample handling, sampling documentation, collection of liquid waste, collection of viscous liquids, sludge collection, collection of pasty materials, preparation sample preparation, sub-sample acquisition, sample packaging and labeling, sample preservation). Preparation of water extract, determination of dry matter content and weight of analytical sample. Separation of liquid and solid phase, analysis of leachate and expression of results.
4. Sampling and analysis of water sample: basic general rules, types of sampling, sample types, sampler, method of sampling of surface, underground, precipitation, waste and drinking water; Requirements for sampling, preservation of samples, transport and storage, adjustment before analysis.
5. Sampling and analysis of soil sample: definition of basic terms, factors and conditions of soil-forming process, soil composition, basic soil properties. Soil sampling. Soil analysis (pH determination, H + content in soil sorption complex by two pH measurements according to Adams and Evans, determination of organically oxidizable carbon). Leach Analysis.
6. Sampling and analysis of mineral samples: Sampling of waste and sample handling. Sequential extraction analysis.
7. Sampling and analysis of air sample: definition of basic terms, factors and conditions necessary for proper sampling, work with field meters.
8. Titration methods, Iodometry (BOD); Chelatometry (Ca + Mg); Neutralization Titration (KNK, ZNK); Argentometry (Cl); Manganometry (COD); Conductometry (determination of acid and base concentration volumetrically and conductometrically). Gravimetric determination: Sulphates, Fe, Ni, Mg, NL.
9. Distillation: Separation of organic substances. Solubility: Transfer sample to solution and structure-solubility relationships. Adsorption: Adsorption properties of activated carbon.
10. Spectophotometry: Mn, PO4, Cr (VI), turbidity, ammoniacal nitrogen.
11. Potentiometry: Determination of pH of samples, ISE.
12. Separation methods: TLC, GC, HPLC.
13. Field practice: Sampling, gas determination (thermally active draining) - use of mobile analytical methods.
14. Field practice: Sampling, determination of physical, chemical and organoleptic parameters (Polanka) - use of mobile analytical methods.
Recommended or Required Reading
Required Reading:
HARVEY, David: Modern Analytical Chemistry 2.0. Electronic Version. 2OO9, 1071 p. ISBN 0–07–237547–7. Dostupné z:
SKOOG, Douglas A. Fundamentals of analytical chemistry. 9th Ed. Belmont, CA: Cengage - Brooks/Cole, 2012. ISBN 978-0-495-55828-6.
FIFIELD, F. W. a D. KEALEY. Principles and practice of analytical chemistry. Malden, MA: Blackwell Science, c2000. ISBN 0-632-05384-4.
PERTILE, E. Instrumental Methods of Analysis. Ostrava: Vysoká škola báňská - Technická univerzita Ostrava, 2017. ISBN 978-80-248-4124-3.
PERTILE, E. a D. SUROVKA. Učební texty k praktiku IMA, multimediální učební text, CD, VŠB TUO 2016. 97 s.
JANKŮ, J., ČERMÁK J.: Vzorkování odpadů. VŠCHT Praha, 1. vyd. 107 s. 2006. Dostupné na
HORÁKOVÁ, Marta. Analytika vody. Vyd. 2., opr. a rozš. Praha: Vydavatelství VŠCHT, 2003, 335 s. ISBN 80-7080-520-x.
HARVEY, David: Modern Analytical Chemistry 2.0. Electronic Version. 2OO9, 1071 p. ISBN 0–07–237547–7. Dostupné z:
Recommended Reading:
KEALEY, D a P HAINES. Analytical chemistry. Oxford: BIOS Scientifc Publishers Limited, 2002, x, 342 s. Instant notes series. ISBN 1859961894.
KENKEL John: Analytical Chemistry for Technicians. Fourth Edition. 2014. 533 p. ISBN 9781566705196 Dostupné z:
PERTILE, E.: Analytická chemie, multimediální učební text, CD FRVŠ/VŠB TUO, 2005. aktualizace 2017.
KOTLÍK, Bohumil, Jan LANGHANS, Pavel BERNÁTH a Vladimír KRAJÁK. Vzorkování. Český Těšín: 2 Theta, 2016. ISBN 978-80-86380-81-0.
HORÁLEK, Vratislav. Vzorkování. Český Těšín: 2 Theta, 2010. ISBN 978-80-86380-53-7.
PERTILE, E. Instrumental Methods of Analysis. Ostrava: Vysoká škola báňská - Technická univerzita Ostrava, 2017. ISBN 978-80-248-4124-3.
Planned learning activities and teaching methods
Experimental work in labs, Terrain work
Assesment methods and criteria
Task TitleTask TypeMaximum Number of Points
(Act. for Subtasks)
Minimum Number of Points for Task Passing
CreditCredit85 (85)46
        Field protocolLaboratory work30 18
        ProtocolLaboratory work30 18
        Final credit testWritten test20 10
        ActivityOther task type5 0