| Course Unit Code | 546-0116/01 |
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| Number of ECTS Credits Allocated | 4 ECTS credits |
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| Type of Course Unit * | Compulsory |
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| Level of Course Unit * | Second Cycle |
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| Year of Study * | First Year |
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| Semester when the Course Unit is delivered | Winter Semester |
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| Mode of Delivery | Face-to-face |
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| Language of Instruction | Czech |
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| Prerequisites and Co-Requisites | There are no prerequisites or co-requisites for this course unit |
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| Name of Lecturer(s) | Personal ID | Name |
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| VOJ195 | doc. Mgr. Hana Vojtková, Ph.D. |
| Summary |
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The subject is focused on consolidation and broadening of microbiological laboratory skills enabling isolation, cultivation and identification of microorganisms from waste and other environmental samples.
Learning outcomes of the subject are to deepen the knowledge and skills of students in working with microorganisms including the application of basic laboratory procedures, methods and techniques used in microbiological analyses of waste samples, including the identification of significant indicator groups of microorganisms and verification of the presence of significant pathogens. |
| Learning Outcomes of the Course Unit |
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Target competences lie in gaining new knowledge and its application in observation, experiments and communication:
- to characterize the morphological characteristics of microorganisms, to understand phylogenetic relations of the different groups, to learn about and to term characteristic organisms of the individual taxons and to understand the significance of pathogenic as well as non-pathogenic species
- to apply the gained knowledge in dealing with practical tasks in seminars, to consider the consequences of a phenomenon from the environmental, sanitary as well as economic points of view; to build a positive attitude to nature
- to be able to work independently on the set tasks, to observe and objectively record the experiment results, to adhere to safe (aseptic) work principles when working with biological material and technical equipment
- to be able to work with expert/academic texts, to be familiar with the expert terminology, to be able to synthetize the obtained experimental data and to justify own approach in dealing with a biological problem |
| Course Contents |
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1. Principles of safe work in a microbiological laboratory
2. Equipment of a microbiological laboratory, basics of aseptic work
3. Preparation of nutrient media and their sterilization, conditions of cultivation of microorganisms
4. Bacteria and their morphological features on nutrient media
5. Microscopic filamentous fungi and their morphology on nutrient media
6. Yeast and yeast microorganisms, their morphological features
7. Cyanobacteria, microscopic algae and their morphology
8. Sampling and preparation of samples for microbiological analysis
9. Cultivation methods for determining the number of microorganisms in a sample
10. Microscopic methods for determining the number of microorganisms in a sample
11. Indirect methods for determining the number of microorganisms in a sample
12. Indicator microorganisms and identification of basic pathogens
13. Other methods of microbiological analysis of waste samples |
| Recommended or Required Reading |
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| Required Reading: |
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MORELLO, Josephine A., Paul A. GRANATO a Helen ECKEL MIZER. Laboratory Manual and Workbook in Microbiology. McGraw-Hill Science, 2003. ISBN 972-0-0724-6354-6
BURDASS, Dariel, John GRAINGER a Janet HURST. Basic Practical Microbiology: A Manual. Society for General Microbiology, 2006. ISBN 0-95368-383-4
GOLDMAN, Emanuel a Lorrence H. GREEN. Practical Handbook of Microbiology. CRC Press, Taylor & Francis Group, 2015. ISBN 978-1-4665-8739-7
KUMAR, Surinder. Textbook of Microbiology. Jaypee Brothers Medical Publishers Ltd., 2012. ISBN 978-93-5025-510-0 |
DEMNEROVÁ, Kateřina a kol. Laboratorní cvičení z mikrobiologie. Praha: Vydavatelství VŠCHT, 2009. ISBN 978-80-7080-415-5
ŘÍHOVÁ-AMBROŽOVÁ, Jana, Dana VEJMELKOVÁ a Pavlína ČIHÁKOVÁ. Technická mikrobiologie a hydrobiologie. Praha: Vydavatelství VŠCHT, 2017. ISBN 978-80-7080-986-0
AMBROŽOVÁ, Jana. Mikroskopické praktikum z hydrobiologie. Praha: Vydavatelství VŠCHT, 2002. ISBN 80-7080-496-3
MORELLO, Josephine A., Paul A. GRANATO a Helen ECKEL MIZER. Laboratory Manual and Workbook in Microbiology. McGraw-Hill Science, 2003. ISBN 972-0-0724-6354-6
VOJTKOVÁ, Hana. Sbírka mikroorganismů: Atlas mikroorganismů. Ostrava: VŠB-TUO, ©2021. Dostupné z: https://www.hgf.vsb.cz/546/cs/veda-a-vyzkum/sbirka-mikroorganismu/ |
| Recommended Reading: |
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BITTON, Gabriel. Wastewater Microbiology. John Wiley & Sons, 2011. ISBN 978-0-470-63033-4
PEPPER,Ian, Charles GERBA a Terry GENTRY. Environmental Microbiology. Academic Press, 2015. ISBN 978-0-123-94626-3
BIOLOG - Microbial Identification & Characterization (www.biolog.com)
List of Prokaryotic Names with Standing in Nomenclature (http://www.bacterio.net)
Species lists in the data base «Species fungorum» (http://www.speciesfungorum.org) |
NĚMEC, Miroslav a Dagmar MATOULKOVÁ. Základy obecné mikrobiologie. Brno: MuniPRESS, 2015. ISBN 978-80-210-7923-6
SEDLÁČEK, Ivo. Taxonomie prokaryot. Brno: Masarykova univerzita, 2007. ISBN 80-210-4207-9
ŘÍHOVÁ-AMBROŽOVÁ, Jana. Mikrobiologie v technologii vod. Praha: Vydavatelství VŠCHT, 2008. ISBN 978-80-7080-676-0
GOLDMAN, Emanuel a Lorrence H. GREEN. Practical Handbook of Microbiology. CRC Press, Taylor & Francis Group, 2015. ISBN 978-1-4665-8739-7 |
| Planned learning activities and teaching methods |
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| Tutorials, Experimental work in labs, Other activities |
| Assesment methods and criteria |
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| Task Title | Task Type | Maximum Number of Points
(Act. for Subtasks) | Minimum Number of Points for Task Passing |
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| Credit | Credit | 100 (100) | 51 |
| Aktivní účast na cvičení | Laboratory work | 50 | 26 |
| Závěrečný test | Written test | 50 | 25 |