| Course Unit Code | 546-0396/04 |
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| Number of ECTS Credits Allocated | 5 ECTS credits |
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| Type of Course Unit * | Compulsory |
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| Level of Course Unit * | First Cycle |
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| Year of Study * | Third Year |
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| Semester when the Course Unit is delivered | Winter Semester |
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| Mode of Delivery | Face-to-face |
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| Language of Instruction | Czech |
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| Prerequisites and Co-Requisites | Course succeeds to compulsory courses of previous semester |
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| Name of Lecturer(s) | Personal ID | Name |
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| PER34 | doc. Mgr. Eva Pertile, Ph.D. |
| Summary |
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| The course is focused on broadening and deepening the knowledge of modern instrumental laboratory techniques, including gaining practical skills. The aim of the course is to provide students with a solid and sufficiently broad theoretical and methodological basis for methods of instrumental analysis, both inorganic and organic. The main focus of the lessons is the interpretation of the theoretical foundations of instrumental methods of analysis. Practical exercises emphasize students' independent approach to solving analytical problems of selected instrumental methods. |
| Learning Outcomes of the Course Unit |
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| Knowledge acquired after the completion of the course: Acquiring basic knowledge of instrumental analytical methods and enhancing students' readiness to solve analytic problems by selected instrumental methods.Skills demonstrated at the end of the course: The student will gain the competence to work in an analytical laboratory with basic instrumental equipment. |
| Course Contents |
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1. Definition of issues. Basic concepts. Information retrieval processes. Analytical methods absolute and comparative. Calibration methods. Developmental trends in instrumental methods of environmental samples analysis.
2. Evaluation of the results of the analysis and the way of their expression with a focus on environmental analysis. Statistical evaluation of analytical results. Reference material, round test. Fundamentals of chemometric approaches and methods.
3. Electroanalytical methods: methods based on potential measurement (equilibrium potentiometry, potentiometric titration). Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
4. Electroanalytical methods: current measurement methods. Voltammetry and polarography: Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
5. Electrogravimetry and coulometry: a constant current and a constant potential. Conductometry. Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
6. Optical analytical methods: theoretical basics. optical spectral devices. Methods without energy-to-radiation exchange (refractometry, polarimetry, nephelometry); Refractometry, Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
7. Emission Atomic Spectral Analysis. Flame photometry. Atomic absorption spectrometry; UV / Vis spectrometry, Luminescence analysis. Fundamentals of theory, instrumentation, qualitative and quantitative aspects. Automatic analyzers with optical detection: FIA, CFA. Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
8. Luminescence analysis. Infrared spectrometry. Raman spectra; X-ray fluorescence spectrometry. Radiochemical methods. Principles of individual methods, instrumentation, applications in environmental component analysis; Advantages, disadvantages.
9. Separation and concentration steps: theory. Separation methods: separation according to principle and according to experimental arrangement, efficiency of the separation process. Extraction. Instrumentation; applications for environmental component analysis; Advantages, disadvantages.
10. Membrane Separation. Mass spectrometry. Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
11. Chromatographic methods. Planar Chromatography: Sorbent Thin Layer Chromatography, Fundamentals of Theory, Instrumentation, Qualitative and Quantitative Aspects, Applications.
12. Column chromatography: GC + HPLC. New Trends: UHPLC, GC / MS, HPLC / MS. Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
13. Electromigration methods: theory, zonal and capillary electrophoresis, carrier electrophoresis and isotachophoresis. Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages. |
| Recommended or Required Reading |
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| Required Reading: |
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PERTILE, E. Instrumental Methods of Analysis. Ostrava: Vysoká škola báňská - Technická univerzita Ostrava, 2017. ISBN 978-80-248-4124-3.
SIVASANKAR, B. Instrumental methods of analysis. New Delhi: Oxford University Press, 2012. ISBN 9780198073918.
BRICKER, B., ed. Experiments in analytical chemistry. London: Auris Reference, c2012. ISBN 978-1-78154-179-1.
CHANG R.: Chemistry. 10th Edition. McGraw-Hill: New York, 2010. 1170 p. Available from: https://archive.org/details/Chemistry_10th_Edition_Raymond_Chang.4-3. |
PERTILE E. a V. ČABLÍK. Instrumentální metody analýzy, ČSVTS VŠB TUO, 2006, 238 s. ISBN 80-248-1049-2.
PERTILE, E. a D. SUROVKA. Učební texty k praktiku IMA, multimediální učební text, CD, VŠB TUO 2016. 97 s.
MELOUN M. a J. MILITKÝ. Kompendium statistického zpracování dat, Academia Praha 2002 (1. vydání), Academia Praha 2006 (2. vydání), Karolinum Praha 2013 (3. vydání). 985 s. Dostupné z: https://meloun.upce.cz/docs/books/kompendium.pdf.
PERTILE, E. Instrumental Methods of Analysis. Ostrava: Vysoká škola báňská - Technická univerzita Ostrava, 2017. ISBN 978-80-248-4124-3. |
| Recommended Reading: |
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LONG, J. H. A textbook of elementary analytical chemistry: qualitative and volumetric. Lexington, KY: [CreateSpace Independent Publishing Platform], 2014. ISBN 978-1-4927-1822-2.
TISSUE, B. M. Basics of analytical chemistry and chemical equilibria. Hoboken: Wiley, c2013. ISBN 978-0-470-59208-3.
CAROLI, S. and G. ZÁRAY, ed. Analytical techniques for clinical chemistry: methods and applications. Hoboken: Wiley, c2012. ISBN 978-0-470-44527-3.
GUARDIA, M. de la a S. GARRIGUES MATEO, ed. Handbook of green analytical chemistry. Chichester: Wiley, 2012. ISBN 978-0-470-97201-4. |
LABUDA, J. Príručka vybraných pojmov v analytickej chémii. Bratislava: Nakladateľstvo STU, 2012. Edícia príručiek. ISBN 9788022737692.
KŘÍŽEK, M. a J. ŠÍMA. Analytická chemie. České Budějovice: Jihočeská univerzita v Českých Budějovicích, Zemědělská fakulta, 2015. ISBN 978-80-7394-486-5.
PRAUS, P. a J. VONTOROVÁ. Analytická chemie II. Ostrava: VŠB - Technická univerzita Ostrava, 2015. ISBN 978-80-248-3734-5. Dostupné z: http://katedry.fmmi.vsb.cz/617/Analyticka_chemie_II.pdf.
JANOŠ, P. Čím se zabývá environmentální analytická chemie. Ústí nad Labem: Univerzita J.E. Purkyně v Ústí nad Labem, Fakulta životního prostředí, 2014. ISBN 978-80-7414-789-0. Dostupné z: http://envimod.fzp.ujep.cz/sites/default/files/skripta/27e_final_tisk.pdf.
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| Planned learning activities and teaching methods |
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| Lectures, Experimental work in labs, Terrain work, Other activities |
| Assesment methods and criteria |
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| Task Title | Task Type | Maximum Number of Points
(Act. for Subtasks) | Minimum Number of Points for Task Passing |
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| Credit and Examination | Credit and Examination | 100 (100) | 51 |
| Credit | Credit | 45 (45) | 18 |
| Protokoly | Laboratory work | 27 | 11 |
| Písemka I | Written test | 5 | 2 |
| Aktivita | Other task type | 3 | 0 |
| Závěrečný zápočtový test | Written test | 10 | 4 |
| Examination | Examination | 55 | 22 |