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Terminated in academic year 2019/2020

Instrumental Methods of Analysis

Type of study Bachelor
Language of instruction Czech
Code 546-0354/03
Abbreviation IMA
Course title Instrumental Methods of Analysis
Credits 8
Coordinating department Department of Environmental Engineering
Course coordinator doc. Mgr. Eva Pertile, Ph.D.

Subject syllabus

1. Definition of the issue. Information-gathering process. The analysis process. Evaluation of analytical results and practices, expression analysis results. Statistical results. Draft action arising from the data obtained. Principles of Good Laboratory Practice. Basics of chemometrics.
2. Sampling, the general principle and sources of the errors. Standard operating procedures for sampling; QA / QC system. Basic parameters sampling of abiotic and biotic matrices.
3. Treatment of the sample before analysis. Nondestructive methods. Destructive methods. Separation and concentration steps.
4. Qualitative analysis: classical system of dividing cations. Evidence heteroatoms in organic compounds.
5. Overview of the basic analytical methods and their general properties: methods based on weighing, measuring volume. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages.
6. An introduction to the instrumental methods. Use of both qualitative and quantitative. Definition of the issue. The analytical methods absolute and comparative. Rapid analytical methods - screening. Intended use. Advantages, disadvantages. Calibration methods. Trends in the instrumental methods of analysis.
7. Electrochemical methods based on the voltage measurement. Electrochemical methods based on the current measurement. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages.
8. Summary separation methods: Planar chromatography: TLC + HPTLC, PC, advantages/disadvantages.
9. Column chromatography: GC + HPLC. New trends: UHPLC, GC / MS, HPLC / MS. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages.
10. Electrophoresis. Mass spectrometry. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages.
11. Overview of the optical methods. Optical spectral device. Refractometry, interferometry, polarimetry and turbidimetry: Principles of the individual methods; instrumentation; application in the analysis of the environmental components; advantages/disadvantages.
12. Atomic emission spectral analysis. Flame photometry. Atomic absorption spectrometry. UV / Vis spectrometry. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages.
13. Luminescence analysis. Infrared spectrometry. Raman spectra; X-ray fluorescence spectrometry. Radiochemical methods. Understanding the various methods, instrumentation, application in the analysis of environmental components; advantages/disadvantages.
14. New trends in instrumental methods with applications in biotechnology.

Literature

CHANG R.: Chemistry. 10th Edition. McGraw-Hill: New York, 2010. 1170 p. Dostupné z: https://archive.org/details/Chemistry_10th_Edition_Raymond_Chang
PERTILE, E. Instrumental Methods of Analysis. Ostrava: Vysoká škola báňská - Technická univerzita Ostrava, 2017. ISBN 978-80-248-4124-3 

Advised literature

ROUESSAC Francis and ROUESSAC, Annick: Chemical Analysis Modern Instrumentation Methods and Techniques. March 2007. 600 pp. ISBN: 978-0-470-85903-2 
NOLLET M., L., Chromatographic Analysis of the Environment, Chromatographic Science Series, Volume 93, 1297 s. 2006, ISBN 0-8247-2629-4