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Terminated in academic year 2015/2016

Instrumental Methods of Analysis

Type of study Bachelor
Language of instruction Czech
Code 546-0354/06
Abbreviation IMA
Course title Instrumental Methods of Analysis
Credits 4
Coordinating department Department of Environmental Engineering
Course coordinator doc. Mgr. Eva Pertile, Ph.D.

Subject syllabus

1. Defining the problem. Basic concepts. Processes of obtaining information. Types of interactions. Chemical equilibrium in analytical chemistry. Appraisal reactions.
2. Process of analysis. Sampling and sample treatment before analysis, separation and concentration steps. Evaluation of analytical results and procedures and expression analysis results. Proposal for action arising from the data obtained. Principles of good laboratory practice.
3. Trends in analytical chemistry - new instrumental procedures, including mobile analytics.
4. Quantitative chemical analysis. The principle of gravimetry, analytical use. Volumetric analysis (volumetry): general procedure for determining, detecting the end point of a titration, titration curves, the calculation of the analyte from the titration data. Volumetric determination of types and their applications.
5. Electroanalytical methods based on voltage measurement: Equilibrium potentiometry - direct potentiometry. Indirect potentiometry. Principles, instrumentation, applications.
6. Electroanalytical methods based on current measurement. Voltammetry and polarography: principles, instrumentation, applications. Electrogravimetry and coulometry: implementing a constant current and constant potential. Conductometry. Principles, instrumentation, applications.
7. Overview of separation methods, principles and their analytical applications: Planar chromatography: thin layer chromatography (TLC + HPTLC), on paper, analytical applications.
8. Column chromatography - a general principle, the distribution of chromium. methods, instrumentation, qualitative analysis, quantitative analysis, chromatogram, analytical use. Characteristic differences in the experimental arrangement and implementation of gas and liquid chromatography. Using chromatographic methods.
9. Extraction - different types of extractions, the balance in the system, the extraction efficiency of the design, analytical applications: LLE, SFE, SPE, SPME. Trends in extraction methods.
10. Electrophoresis - principles, instrumentation, analytical applications: IEF, CE, isotachophoresis. Membrane separation: ultrafiltration, dialysis, reverse osmosis, electrodialysis. Mass spectrometry - principles of methods, instrumentation, mass spectrum, analytical use.
11. Overview of optical methods. Optical spectral apparatus. Refractometry, interferometry, polarimetry, nephelometry and turbidimetry: principles, instrumentation and analytical applications.
12. Atomic emission spectral analysis. Flame photometry. Atomic absorption spectrometry. UV / Vis spectroscopy, LED analysis. Principle of the method, instrumentation, analytical use.
13. Infrared spectroscopy. Raman spectrum - the principle of the method, analytical recovery.
14. X-ray fluorescence spectrometry - principles, instrumentation, analytical use. Radiochemical methods.

Literature

CHANG R.: Chemistry. 10th Edition. McGraw-Hill: New York, 2010. 1170 p. Dostupné z: https://archive.org/details/Chemistry_10th_Edition_Raymond_Chang
PERTILE, E. Instrumental Methods of Analysis. Ostrava: Vysoká škola báňská - Technická univerzita Ostrava, 2017. ISBN 978-80-248-4124-3 

Advised literature

ROUESSAC Francis and ROUESSAC, Annick: Chemical Analysis Modern Instrumentation Methods and Techniques. March 2007. 600 pp. ISBN: 978-0-470-85903-2 
NOLLET M., L., Chromatographic Analysis of the Environment, Chromatographic Science Series, Volume 93, 1297 s. 2006, ISBN 0-8247-2629-4