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Terminated in academic year 2017/2018

Biochemistry

Type of study Bachelor
Language of instruction Czech
Code 546-0371/01
Abbreviation BIO
Course title Biochemistry
Credits 3
Coordinating department Department of Environmental Engineering
Course coordinator prof. Ing. Daniela Plachá, Ph.D.

Subject syllabus

1. Introduction. Description of biochemistry and sphere of research. Composition and organization of living matter. Basic unit of living organisms, biopolymers, principles of metabolisms and energy transformation.
2. Peptides and proteins. Amino acides, structure, chemical and physical properties, chirality, importance of amino acides, isolation and analysis of amino acides.
3. Peptides and proteins. Structure, sequences and their determination, biochemical significant peptides. Strukture of proteins, conformation and their changes, allostery.
Classification of proteins. Isolation of proteins. Methods of protein research.
4. Nucleic acids. Nukleotides. Composition and structure of DNA and RNA,
properties, methods of research.
5. Saccharides. Properties, classification of monosaccharides. Monosaccharides and their derivatives, di-, oligo- and polysaccharides. Structures, chirality
and function in living organisms. Glycosides. Glycoproteins and proteoglycans. Analysis of
saccharides.
6. Lipids. Compositions, properties, function in organisms. Neutral
lipids, waxes, polar lipids. Steroides. Structure and significancy of biomembranes.
7. Enzymes. Structure, confirmation, properties, classification, nomenclature.
Occurence of enzymes. Activity. Coenzymes. Applied enzymology.
8. Mechanisms and kinetics of enzymatic reactions. Factors influencing of enzymatic reactions. Inhibitors. Other biocatalyzators.
9. Metabolisms and energy transformations in living systems. Catabolism and anabolism. Biochemical reactions, bioenergy.
10. Respiratory chain and a oxidative fosforylation. Photosynthesis. Transfer of
electrons and protons in respiratory chain. Biological oxidation. Krebs cycle. Glyoxalate cycle.
11. Metabolisms of saccharides and lipids. Glykolysis. Fermentation.
Metabolisms of lipids. Metabolisms of glycerol. Biosynthesis of lipids.
12. Metabolisms of proteins, nucleic acids, and other nitroegen-containig compounds. Degradation of amino acides. Biosynthesis amino acides.
13. Biochemistry of microorganisms. Dividing according of carbon and energy source.
Microorganisms in environment. Influence of the environment on living organisms. Cycles of biogenic elements in nature. Biodegradation, biosorption.
14.-15. Application of biochemistry in technologies. Food and alimentary, biotechnology,
formation of biogas, treatment of minerals, waste disposal.

Literature

CH. K. Mathews, K. E. van Holde, K. G. Ahern. Biochemistry. Prentice Hall; 3 edition 1999.
D. L. Nelson, M. M. Cox. Lehninger Principles of Biochemistry, Fourth Edition, W. H. Freeman, 2008.

Advised literature

Nelson, D.L., Cox, M.M. Lehninger Principles of Biochemistry. W. H. Freeman, 2012.
Campbell M., Farrell S. Biochemistry. 2007.