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Terminated in academic year 2020/2021

Laboratory Practicum

Type of study Follow-up Master
Language of instruction Czech
Code 546-0844/01
Abbreviation LP
Course title Laboratory Practicum
Credits 5
Coordinating department Department of Environmental Engineering
Course coordinator doc. Mgr. Eva Pertile, Ph.D.

Subject syllabus

1. Introduction, work safety, laboratory glassware + utilities.
2. Basic laboratory work (weighing, precipitation, filtration, drying, titration, distillation, etc.); calculations: solution composition, titration factor, volumetric factor, pH value, basic statistical parameters.
3. Sampling and analysis of the waste sample: Sampling and sampling (preparatory part, sampling and laboratory sample preparation, sample handling, sampling documentation, liquid waste collection, viscous liquids collection, sludge collection, paste-like material picking, preparation sample mix, sample subtraction procedure, sample packaging and labeling, preservation of the sample). Preparation of aqueous extraction, determination of dry matter content and weight of analytical sample. Separation of liquid and solid phases, analysis of leachate and expression of results (according to methods for water-pH analysis, conductivity, ecotoxicity, DOC, phenolic index, ammonium ions, nitrates, nitrates, fluorides, chlorides, sulphates, Fe, Hg, Mn, Ni, Pb, Se, Zn).
4. Sampling and analysis of water samples: basic general rules, types of sampling, sample types, sampler, method of taking surface, underground, precipitation, wastewater and drinking water; sampling requirements, preservation of samples, transport and storage, pre-analysis. Drinking water analysis (basic concepts, microbiological and biological indicators, physical, chemical and organoleptic indicators). Surface water analysis (basic concepts, immission standards for indicators of permissible surface water pollution, ČSN 75 7221 classification of surface water quality). Basic water analyzes (taste, smell, color, temperature, transparency, turbidity, NK, COD, BOD, chlorine, ammoniacal nitrogen, nitrates, nitrates, Ca + Mg, Sulphates, Chlorides, phosphates, conductivity, pH etc.).
5. Soil sampling and analysis: Determination of basic concepts, factors and conditions of soil formation, soil composition, basic soil properties (color, structure, granularity, soil water, consistency, oxidation-reduction potential, nutrient content, humus content, soil sorption capacity, specific and bulk density, biological properties of soils). Sampling of soils (sampling procedure, adjustment of the volume of quaternary samples). Soil analysis (pH determination, H + fraction in sorption)
Soil complex by double pH measurement by Adam and Evans, determination of organically oxidizable carbon). Liquid analysis (determination of phosphates, Fe, Al, Ca, Mg, toxic metals - titration, colorimetry, AAS).
6. Titration methods: Jodometry (BOD, SAVO); Chelation (Ca + Mg); Neutralization titration (KNK, ZNK); Argentometry (Cl); Manganometry (COD); Conductometry (determination of acid and volumetric and conductivity).
7. Gravimetric determination: Sulphates, Fe, Ni, Mg, NL.
8. Distillation: Separation of organic substances, density of alcohol.
9. Solubility: Sample transfer into solution and relationships between structure and solubility.
10. Adsorption: Adsorption properties of activated carbon.
11. Spectrophotometry: Mn, PO4, Cr (VI), Turbidity, ammoniacal nitrogen.
12. Potentiometry: Determination of pH of samples, ISE.
13. Separation methods: TLC, GC, HPLC.
14. Field practice: Sampling, determination of physical, chemical and organoleptic indicators (Polanka) - use of mobile analytical methods.

Literature

HARVEY, David: Modern Analytical Chemistry 2.0. Electronic Version. 2OO9, 1071 p. ISBN 0–07–237547–7. Dostupné z: http://acad.depauw.edu/harvey_web/eText%20Project/pdf%20file/AnalChem2.0.pdf.
SKOOG, Douglas A. Fundamentals of analytical chemistry. 9th Ed. Belmont, CA: Cengage - Brooks/Cole, 2012. ISBN 978-0-495-55828-6 .
FIFIELD, F. W. a D. KEALEY. Principles and practice of analytical chemistry. Malden, MA: Blackwell Science, c2000. ISBN 0-632-05384-4 .
PERTILE, E. Instrumental Methods of Analysis. Ostrava: Vysoká škola báňská - Technická univerzita Ostrava, 2017. ISBN 978-80-248-4124-3 .

Advised literature

KEALEY, D a P HAINES. Analytical chemistry. Oxford: BIOS Scientifc Publishers Limited, 2002, x, 342 s. Instant notes series. ISBN 1859961894.
KENKEL John: Analytical Chemistry for Technicians. Fourth Edition. 2014. 533 p. ISBN 9781566705196  Dostupné z: http://www.slideshare.net/RonaldTalvat/analytical-chemistry-for-technicians-4th-edition.