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Terminated in academic year 2018/2019

Instrumental methods of analysis II

Type of study Doctoral
Language of instruction Czech
Code 9360-0903/01
Abbreviation MIA
Course title Instrumental methods of analysis II
Credits 10
Coordinating department CNT - Nanotechnology Centre
Course coordinator prof. Ing. Daniela Plachá, Ph.D.

Subject syllabus

Theory of chromatografic processes.
Gas chromatography – instrumentation, stacionary phase, detectors, optimization of separation, reactionary gas chromatography, sample derivatization vzorků, GCxGC analysis, applications.
Liquid chromatography – instrumentation, stacionary and mobile phase, interaction in liquid chromatography, detectors, optimization of separation, applications.
Supercritical fluid chromatography – theory of supercritical liquids, mobile and stacionary phasese, optimization of separation, applications.
Mass spectromery – theory, instrumentation, mass spectra, applications.
Gas chromatography in connection with mass spectrometry – ionization techniques, ion separation (kvadrupol detectors, ion trap, TOF), optimization of processes, applications. Methods of MS/MS.
Liquid chromatography in connection with mass spectrometry – ionization techniques, ion separation, optimization of mass detector parameters, applications. Methods of MS/MS.
Practical part:
Development and optimization of separation method, validation of methods, measurement uncertainety, evaluation of chromatographic patterns.

Literature

• McMASTER, M. C. GC/MS. Sec. Ed. Hoboken, USA: John Wiley & Sons, Inc., 2007.
• McMASTER, M. C. LC/MS, New York: John Wiley&Sons, 2005.
• McMAHON, G. Analytical Instrumentation, New York: John Wiley&Sons, 2007.
• SPARKMAN, O.D., Penton, Z.E., KITSON, F.G. Gas Chromatography and Mass Spectrometry,
Sed. Ed. Oxford: Elsevier, 2011.
• LUNDANES, E., REUBSAET, L., GREIBROKK, T. Chromatography: Basic Principles, Sample
Preparations and Related Methods, New York: John Wiley & Sons, 2013.
• ŠTULÍK, K., ŠEVČÍK, J., PACÁKOVÁ, V., JELÍNEK, I., COUFAL, P., BOSÁKOVÁ, Z.
Analytické separační metody, Praha: Nakladatelství Karolinum, 2005.

Advised literature

• McMASTER, M. C. GC/MS. Sec. Ed. Hoboken, USA: John Wiley & Sons, Inc., 2007.
• McMASTER, M. C. LC/MS, New York: John Wiley&Sons, 2005.
• McMAHON, G. Analytical Instrumentation, New York: John Wiley&Sons, 2007.
• SPARKMAN, O.D., Penton, Z.E., KITSON, F.G. Gas Chromatography and Mass Spectrometry,
Sed. Ed. Oxford: Elsevier, 2011.
• LUNDANES, E., REUBSAET, L., GREIBROKK, T. Chromatography: Basic Principles, Sample
Preparations and Related Methods, New York: John Wiley & Sons, 2013.


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