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Microbiology Practicum

Type of study Follow-up Master
Language of instruction English
Code 546-0116/02
Abbreviation MBP
Course title Microbiology Practicum
Credits 4
Coordinating department Department of Environmental Engineering
Course coordinator doc. Mgr. Hana Vojtková, Ph.D.

Subject syllabus

1. Principles of safe work in a microbiological laboratory
2. Equipment of a microbiological laboratory, basics of aseptic work
3. Preparation of nutrient media and their sterilization, conditions of cultivation of microorganisms
4. Bacteria and their morphological features on nutrient media
5. Microscopic filamentous fungi and their morphology on nutrient media
6. Yeast and yeast microorganisms, their morphological features
7. Cyanobacteria, microscopic algae and their morphology
8. Sampling and preparation of samples for microbiological analysis
9. Cultivation methods for determining the number of microorganisms in a sample
10. Microscopic methods for determining the number of microorganisms in a sample
11. Indirect methods for determining the number of microorganisms in a sample
12. Indicator microorganisms and identification of basic pathogens
13. Other methods of microbiological analysis of waste samples

Literature

MORELLO, Josephine A., Paul A. GRANATO a Helen ECKEL MIZER. Laboratory Manual and Workbook in Microbiology. McGraw-Hill Science, 2003. ISBN 972-0-0724-6354-6
BURDASS, Dariel, John GRAINGER a Janet HURST. Basic Practical Microbiology: A Manual. Society for General Microbiology, 2006. ISBN 0-95368-383-4 
GOLDMAN, Emanuel a Lorrence H. GREEN. Practical Handbook of Microbiology. CRC Press, Taylor & Francis Group, 2015. ISBN 978-1-4665-8739-7 
KUMAR, Surinder. Textbook of Microbiology. Jaypee Brothers Medical Publishers Ltd., 2012. ISBN 978-93-5025-510-0 

Advised literature

BITTON, Gabriel. Wastewater Microbiology. John Wiley & Sons, 2011. ISBN 978-0-470-63033-4 
PEPPER,Ian, Charles GERBA a Terry GENTRY. Environmental Microbiology. Academic Press, 2015. ISBN 978-0-123-94626-3 
BIOLOG - Microbial Identification & Characterization (www.biolog.com)
List of Prokaryotic Names with Standing in Nomenclature (http://www.bacterio.net)
Species lists in the data base «Species fungorum» (http://www.speciesfungorum.org)


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