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Instrumental Methods of Analysis II

Type of study Bachelor
Language of instruction English
Code 546-0396/05
Abbreviation IMA II
Course title Instrumental Methods of Analysis II
Credits 5
Coordinating department Department of Environmental Engineering
Course coordinator doc. Mgr. Eva Pertile, Ph.D.

Subject syllabus

1. Definition of issues. Basic concepts. Information retrieval processes. Analytical methods absolute and comparative. Calibration methods. Developmental trends in instrumental methods of environmental samples analysis.
2. Evaluation of the results of the analysis and the way of their expression with a focus on environmental analysis. Statistical evaluation of analytical results. Reference material, round test. Fundamentals of chemometric approaches and methods.
3. Electroanalytical methods: methods based on potential measurement (equilibrium potentiometry, potentiometric titration). Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
4. Electroanalytical methods: current measurement methods. Voltammetry and polarography: Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
5. Electrogravimetry and coulometry: a constant current and a constant potential. Conductometry. Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
6. Optical analytical methods: theoretical basics. optical spectral devices. Methods without energy-to-radiation exchange (refractometry, polarimetry, nephelometry); Refractometry, Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
7. Emission Atomic Spectral Analysis. Flame photometry. Atomic absorption spectrometry; UV / Vis spectrometry, Luminescence analysis. Fundamentals of theory, instrumentation, qualitative and quantitative aspects. Automatic analyzers with optical detection: FIA, CFA. Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
8. Luminescence analysis. Infrared spectrometry. Raman spectra; X-ray fluorescence spectrometry. Radiochemical methods. Principles of individual methods, instrumentation, applications in environmental component analysis; Advantages, disadvantages.
9. Separation and concentration steps: theory. Separation methods: separation according to principle and according to experimental arrangement, efficiency of the separation process. Extraction. Instrumentation; applications for environmental component analysis; Advantages, disadvantages.
10. Membrane Separation. Mass spectrometry. Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
11. Chromatographic methods. Planar Chromatography: Sorbent Thin Layer Chromatography, Fundamentals of Theory, Instrumentation, Qualitative and Quantitative Aspects, Applications.
12. Column chromatography: GC + HPLC. New Trends: UHPLC, GC / MS, HPLC / MS. Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
13. Electromigration methods: theory, zonal and capillary electrophoresis, carrier electrophoresis and isotachophoresis. Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.

Literature

PERTILE, E. Instrumental Methods of Analysis. Ostrava: Vysoká škola báňská - Technická univerzita Ostrava, 2017. ISBN 978-80-248-4124-3 .
SIVASANKAR, B. Instrumental methods of analysis. New Delhi: Oxford University Press, 2012. ISBN 9780198073918 .
BRICKER, B., ed. Experiments in analytical chemistry. London: Auris Reference, c2012. ISBN 978-1-78154-179-1 .
CHANG R.: Chemistry. 10th Edition. McGraw-Hill: New York, 2010. 1170 p. Available from: https://archive.org/details/Chemistry_10th_Edition_Raymond_Chang.4-3.

Advised literature

LONG, J. H. A textbook of elementary analytical chemistry: qualitative and volumetric. Lexington, KY: [CreateSpace Independent Publishing Platform], 2014. ISBN 978-1-4927-1822-2 .
TISSUE, B. M. Basics of analytical chemistry and chemical equilibria. Hoboken: Wiley, c2013. ISBN 978-0-470-59208-3 .
CAROLI, S. and G. ZÁRAY, ed. Analytical techniques for clinical chemistry: methods and applications. Hoboken: Wiley, c2012. ISBN 978-0-470-44527-3 .
GUARDIA, M. de la a S. GARRIGUES MATEO, ed. Handbook of green analytical chemistry. Chichester: Wiley, 2012. ISBN 978-0-470-97201-4 .