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Laboratory Practicum

Type of study Follow-up Master
Language of instruction English
Code 546-0844/03
Abbreviation LP
Course title Laboratory Practicum
Credits 4
Coordinating department Department of Environmental Engineering
Course coordinator doc. Mgr. Eva Pertile, Ph.D.

Osnova předmětu

1. Basic laboratory work; calculations: composition of solutions, titration factor, volumetric factor, pH value, basic statistical parameters.
2. Sampling and analysis of waste sample: Sampling of waste and sample handling (preparation part, sampling and preparation of laboratory sample, sample handling, sampling documentation, collection of liquid waste, collection of viscous liquids, sludge collection, collection of pasty materials, preparation sample preparation, sub-sample acquisition, sample packaging and labeling, sample preservation). Preparation of water extract, determination of dry matter content and weight of analytical sample. Separation of liquid and solid phase, analysis of leachate and expression of results.
3. Sampling and analysis of water sample: basic general rules, types of sampling, sample types, sampler, method of sampling of surface, underground, precipitation, waste and drinking water; Requirements for sampling, preservation of samples, transport and storage, adjustment before analysis.
4. Sampling and analysis of soil sample: definition of basic terms, factors and conditions of soil-forming process, soil composition, basic soil properties. Soil sampling. Soil analysis (pH determination, H + content in soil sorption complex by two pH measurements according to Adams and Evans, determination of organically oxidizable carbon). Leach Analysis.
5. Sampling and analysis of mineral samples: Sampling of waste and sample handling. Sequential extraction analysis.
6. Sampling and analysis of air sample: definition of basic terms, factors and conditions necessary for proper sampling, work with field meters.
7. Titration methods, Iodometry (BOD); Chelatometry (Ca + Mg); Neutralization Titration (KNK, ZNK); Argentometry (Cl); Manganometry (COD); Conductometry (determination of acid and base concentration volumetrically and conductometrically). Gravimetric determination: Sulphates, Fe, Ni, Mg, NL.
8. Distillation: Separation of organic substances. Solubility: Transfer sample to solution and structure-solubility relationships. Adsorption: Adsorption properties of activated carbon.
9. Spectophotometry: Mn, PO4, Cr (VI), turbidity, ammoniacal nitrogen.
10. Potentiometry: Determination of pH of samples, ISE.
11. Separation methods: TLC, GC, HPLC.
12. Field practice: Sampling, gas determination (thermally active draining) - use of mobile analytical methods.
13. Field practice: Sampling, determination of physical, chemical and organoleptic parameters (Polanka) - use of mobile analytical methods.

Povinná literatura

HARVEY, David: Modern Analytical Chemistry 2.0. Electronic Version. 2OO9, 1071 p. ISBN 0–07–237547–7. Dostupné z: http://acad.depauw.edu/harvey_web/eText%20Project/pdf%20file/AnalChem2.0.pdf.
SKOOG, Douglas A. Fundamentals of analytical chemistry. 9th Ed. Belmont, CA: Cengage - Brooks/Cole, 2012. ISBN 978-0-495-55828-6 .
FIFIELD, F. W. a D. KEALEY. Principles and practice of analytical chemistry. Malden, MA: Blackwell Science, c2000. ISBN 0-632-05384-4 .
PERTILE, E. Instrumental Methods of Analysis. Ostrava: Vysoká škola báňská - Technická univerzita Ostrava, 2017. ISBN 978-80-248-4124-3 .

Doporučená literatura

KEALEY, D a P HAINES. Analytical chemistry. Oxford: BIOS Scientifc Publishers Limited, 2002, x, 342 s. Instant notes series. ISBN 1859961894.
KENKEL John: Analytical Chemistry for Technicians. Fourth Edition. 2014. 533 p. ISBN 9781566705196  Dostupné z: http://www.slideshare.net/RonaldTalvat/analytical-chemistry-for-technicians-4th-edition.