| Course Unit Code | 651-2053/01 |
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| Number of ECTS Credits Allocated | 1 ECTS credits |
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| Type of Course Unit * | Compulsory |
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| Level of Course Unit * | First Cycle |
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| Year of Study * | Second Year |
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| Semester when the Course Unit is delivered | Winter Semester |
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| Mode of Delivery | Face-to-face |
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| Language of Instruction | Czech |
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| Prerequisites and Co-Requisites | Course succeeds to compulsory courses of previous semester |
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| Name of Lecturer(s) | Personal ID | Name |
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| MAJ0149 | Mgr. Mária Majeská Čudejková, Ph.D. |
| Summary |
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| The course provides basic information about nucleic acids DNA and RNA, and introduction to basic mechanisms of molecular biology - replication, transcription and translation. Students will learn the basic methods of molecular biology used for the analysis of nucleic acids or proteins and their practical applications. |
| Learning Outcomes of the Course Unit |
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| Obtaining fundamental knowledge in the field of molecular biology. Competences acquired: ability to define main concepts and to describe main approaches used in molecular and cell biology. |
| Course Contents |
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| Information macromolecules, structure of DNA and RNA. Molecular structure of genes and chromosomes. Replication of DNA, comparison of the replication in prokaryotes and eukaryotes. Errors and repairs of DNA. Transcription. Principles of the control of gene expression in prokaryotes and eukaryotes. Regulatory sequences, activators and repressors. Processing of eukaryotic mRNA. Translation. Structure of ribosomes. Genetic code and its importance. Structure of tRNA. Activation of amino acids. Initiation of the translation in prokaryotes and eukaryotes. Elongation. Termination. Posttranslational modifications of proteins. Chaperones and chaperonines. Fundamental methods of molecular biology. Isolation of DNA and RNA. Electrophoretic analysis of nucleic acids. Restriction analysis of DNA, construction of restriction maps, genome mapping. Polymerase chain reaction (PCR) in vitro. DNA sequencing, sequencing of genomes. Northern and Southern hybridization. Cloning strategies, transfer of DNA to bacterial and eukaryotic cells. Preparation of recombinant proteins. Site-directed mutagenesis. Transgenic organisms. |
| Recommended or Required Reading |
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| Required Reading: |
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VOET, Donald a Judith G. VOET. Biochemistry. 4th ed. Hoboken, N.J.: John Wiley, c2011. ISBN 978-0-470-57095-1.
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ALBERTS, Bruce, Alexander JOHNSON, Julian LEWIS, et al. Molecular biology of the cell. Sixth edition. New York: Garland Science, 2015. ISBN 978-0-8153-4464-3.
RUML, Tomáš, Michaela RUMLOVÁ a Václav PAČES. Genové inženýrství. Praha: Vysoká škola chemicko-technologická, 2007. ISBN 978-80-7080-499-5.
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| Recommended Reading: |
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VOET, Donald and Judith G. VOET. Biochemistry. 4th ed. Hoboken, N.J.: John Wiley, c2011. ISBN 978-0-470-57095-1.
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VOET, Donald a Judith G. VOET. Biochemistry. 4th ed. Hoboken, N.J.: John Wiley, c2011. ISBN 978-0-470-57095-1.
BROWN, T. A. Klonování genů a analýza DNA: úvod. Olomouc: Univerzita Palackého v Olomouci, 2007. ISBN 9788024417196.
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| Planned learning activities and teaching methods |
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| Lectures |
| Assesment methods and criteria |
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| Task Title | Task Type | Maximum Number of Points
(Act. for Subtasks) | Minimum Number of Points for Task Passing |
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| Examination | Examination | 100 | 51 |