| Course Unit Code | 651-2061/01 |
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| Number of ECTS Credits Allocated | 3 ECTS credits |
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| Type of Course Unit * | Compulsory |
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| Level of Course Unit * | First Cycle |
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| Year of Study * | Third Year |
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| Semester when the Course Unit is delivered | Winter Semester |
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| Mode of Delivery | Face-to-face |
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| Language of Instruction | Czech |
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| Prerequisites and Co-Requisites | Course succeeds to compulsory courses of previous semester |
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| Name of Lecturer(s) | Personal ID | Name |
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| FRE0040 | doc. RNDr. Jitka Frébortová, Ph.D. |
| Summary |
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| Molecular biotechnology is a modern and dynamic field that exploits possibilities of manipulation with genetic information of the organism. The course describes experimental strategies leading to the expression of functional genes in prokaryotic and eukaryotic cells and to the production of authentic proteins useful as human therapeutics. The use of cell systems, especially microbial, as “biological factories” for the production of therapeutics, pharmaceutical proteins and nucleic acids, chemical compounds, antibiotics and biopolymers and the use of genetically modified microorganisms for biodegradation, biomass processing and agriculture are also demonstrated. |
| Learning Outcomes of the Course Unit |
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| To introduce modern industrial, biomedical, pharmaceutical and agricultural applications of microbial and cell cultures. Competences acquired: student should be able to describe basic principles of molecular biotechnology, to describe principles of production of commercial products and therapeutics using genetically engineered microorganisms and cell cultures, and to describe the use of genetically engineered microorganisms for insect pest control, remediation and biomass utilization. |
| Course Contents |
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1. Subject of molecular biotechnology. Microbial diversity and microorganisms used in biotechnology.
2. Molecular cloning.
3. Protein production in prokaryotic cells.
4. Protein production in eukaryotic cells. Part I: general characteristics of eukaryotic expression systems, yeast.
5. Protein production in eukaryotic cells. Part II: baculovirus, mammalian cells.
6. Protein engineering.
7. Large-scale production of proteins from recombinant microorganisms.
8. Molecular diagnostics.
9. Protein therapeutics. Nucleic acids as therapeutics.
10. Vaccines. Synthesis of enzymes, small biological molecules, antibiotics and biopolymers using recombinant microorganisms.
11. Bioremediation and biomass utilization.
12. Microbial insecticides.
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| Recommended or Required Reading |
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| Required Reading: |
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| GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology: Principles and applications of recombinant DNA, 4th Edition. Washington: ASM Press, USA, c2010. ISBN 978-1-55581-498-4. |
| GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology: Principles and applications of recombinant DNA, 4th Edition. Washington: ASM Press, USA, c2010. ISBN 978-1-55581-498-4. |
| Recommended Reading: |
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| Comprehensive biotechnology. Volume 1-6. 2nd ed. Editor Murray MOO-YOUNG. Amsterdam: Elsevier, c2011. ISBN 9780080885049. |
| Comprehensive biotechnology. Volume 1-6. 2nd ed. Editor Murray MOO-YOUNG. Amsterdam: Elsevier, c2011. ISBN 9780080885049. |
| Planned learning activities and teaching methods |
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| Lectures, Other activities |
| Assesment methods and criteria |
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| Task Title | Task Type | Maximum Number of Points
(Act. for Subtasks) | Minimum Number of Points for Task Passing |
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| Examination | Examination | 100 | 51 |