| Course Unit Code | 651-2064/01 |
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| Number of ECTS Credits Allocated | 5 ECTS credits |
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| Type of Course Unit * | Compulsory |
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| Level of Course Unit * | First Cycle |
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| Year of Study * | Third Year |
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| Semester when the Course Unit is delivered | Summer Semester |
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| Mode of Delivery | Face-to-face |
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| Language of Instruction | Czech |
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| Prerequisites and Co-Requisites | Course succeeds to compulsory courses of previous semester |
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| Name of Lecturer(s) | Personal ID | Name |
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| KME0022 | Ing. Štěpán Kment, Ph.D. |
| Summary |
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| The course aims to practice the knowledge of students acquired in the courses Physical-chemical methods of studying materials and some selected techniques discussed in the course Instrumental Analysis. This course is scheduled in the summer semester of the third year and is the last course focused on the characterization of materials, so the emphasis is on consolidating knowledge related to the discussed experimental techniques, including practical verification of their use in areas related to environmental technologies. Students will get acquainted with the following methods: determination of particle size distribution by selected techniques, methods of determination of specific surface area and pore size distribution, IR and Raman spectroscopy. The course will also demonstrate and practice techniques of XPS, AFM microscopy and Mössbauer spectroscopy, which, although not widely used in operating laboratories, have their undoubted importance in the field of material characterization and thus broaden the horizons of students in this study program. The task of determining the in vitro toxicity of nanomaterials using flow cytometry is also included among the laboratory exercises. Two exercises in the end are then focused on demonstrating a comprehensive approach to the analysis of water and waste materials, the last exercise is devoted to student presentations. |
| Learning Outcomes of the Course Unit |
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| The students will get acquainted with numerous techniques used for the purpose of the material and solid sample analysis. |
| Course Contents |
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Practical excercises:
1. Introductory lesson, work safety, acquaintance with practiced topics, acquaintance with laboratories.
2. Size characterization of powder materials using laser diffraction and dynamic light scattering method.
3. Use of transmission electron microscopy for size, morphological and structural characterization of materials.
4. Size and morphological characterization of nanoparticles using atomic force microscopy.
5. Determination of surface area and pore size distribution of nanocrystalline and porous materials.
6. IR spectroscopy in the characterization of some surface-modified nanoparticles - data processing, evaluation
7. Raman spectroscopy in the characterization of some surface-modified nanoparticles - data processing, evaluation.
8. Flow cytometry in the study of in vitro toxicity of nanomaterials. (introduction to nanotoxicology, study of the effect of nanomaterials on living systems, determination of cell viability by the Live / Dead method, determination of the effect of nanomaterials on the cell cycle).
9. Modern techniques of studying the chemical composition of sample surfaces - XPS, zeta potential, atomic force microscopy
10. Determination of chemical and physical parameters of samples in the liquid state (analysis of chemical composition, use of electrochemical methods in water analysis, etc.)
11. Analysis of industrial waste (treatment and preparation of samples for analysis, determination of basic parameters such as loss on drying and annealing, determination of chemical and phase composition,…).
12. Choice of procedure for characterization of unknown material, selection of methods and their principles, justification of the selection - presentation of students.
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| Recommended or Required Reading |
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| Required Reading: |
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KUMAR, Challa S.S.R., In-situ Characterization Techniques for Nanomaterials, 1st Edition, Springer, [2018]. ISBN 9783662563212.
KAFLÉ, Bhim Prasad, Chemical Analysis and Material Characterization by Spectrophotometry, Elsevier, [2019]. ISBN 9780128148662. |
KUMAR, Challa S.S.R., In-situ Characterization Techniques for Nanomaterials, 1st Edition, Springer, [2018]. ISBN 9783662563212.
KAFLÉ, Bhim Prasad, Chemical Analysis and Material Characterization by Spectrophotometry, Elsevier, [2019]. ISBN 9780128148662.
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| Recommended Reading: |
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| DE OLIVEIRA Osvlado Novais, Jr., M. FERREIRA, F. DE LIMA LEITE, A.L. DA RÓZ, eds. Nanocharacterization Techniques, William Andrew Publishing, [2017]. ISBN 9780323497787. |
DE OLIVEIRA Osvlado Novais, Jr., M. FERREIRA, F. DE LIMA LEITE, A.L. DA RÓZ, eds. Nanocharacterization Techniques, William Andrew Publishing, [2017]. ISBN 9780323497787.
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| Planned learning activities and teaching methods |
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| Experimental work in labs |
| Assesment methods and criteria |
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| Task Title | Task Type | Maximum Number of Points
(Act. for Subtasks) | Minimum Number of Points for Task Passing |
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| Graded credit | Graded credit | 100 | 51 |