| Course Unit Code | 546-0354/07 |
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| Number of ECTS Credits Allocated | 5 ECTS credits |
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| Type of Course Unit * | Compulsory |
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| Level of Course Unit * | First Cycle |
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| Year of Study * | Third Year |
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| Semester when the Course Unit is delivered | Winter Semester |
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| Mode of Delivery | Face-to-face |
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| Language of Instruction | Czech |
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| Prerequisites and Co-Requisites | Course succeeds to compulsory courses of previous semester |
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| Name of Lecturer(s) | Personal ID | Name |
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| PER34 | doc. Mgr. Eva Pertile, Ph.D. |
| Summary |
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| The lecture provides information about selected modern instrumental methods of research and monitoring of the environment, its use and application. During the lectures, students will get acquainted with the principles of analytical methods that are commonly used in control and development analytical laboratories. |
| Learning Outcomes of the Course Unit |
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Obtaining basic knowledge not only about environmental sampling in relation to its share in the overall uncertainty of environmental analyzes but also in instrumental analytical methods. This will increase student readiness to solve analytic problems by selected instrumental methods.
Obtaining the competence to work in an analytical laboratory with basic instrumental equipment, the ability to present and evaluate the results, to learn the correct principles of working with the sample. |
| Course Contents |
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1. Definition of the issue. Information-gathering process. The analysis process. Evaluation of analytical results and practices, expression analysis results. Statistical results. Draft action arising from the data obtained. Principles of Good Laboratory Practice. Basics of chemometrics.
2. Sampling, the general principle and sources of the errors. Standard operating procedures for sampling; QA / QC system. Basic parameters sampling of abiotic and biotic matrices.
3. Treatment of the sample before analysis. Nondestructive methods. Destructive methods. Separation and concentration steps.
4. Qualitative analysis: classical system of dividing cations. Evidence heteroatoms in organic compounds.
5. Overview of the basic analytical methods and their general properties: methods based on weighing, measuring volume. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages.
6. Electrochemical methods based on the voltage measurement. Electrochemical methods based on the current measurement. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages.
7. Summary separation methods: Planar chromatography: TLC + HPTLC, PC, advantages/disadvantages.
8. Column chromatography: GC + HPLC. New trends: UHPLC, GC / MS, HPLC / MS. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages.
9. Electrophoresis. Mass spectrometry. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages.
10. Overview of the optical methods. Optical spectral device. Refractometry, interferometry, polarimetry and turbidimetry: Principles of the individual methods; instrumentation; application in the analysis of the environmental components; advantages/disadvantages.
11. Atomic emission spectral analysis. Flame photometry. Atomic absorption spectrometry. UV / Vis spectrometry. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages.
12. Luminescence analysis. Infrared spectrometry. Raman spectra; X-ray fluorescence spectrometry. Radiochemical methods. Understanding the various methods, instrumentation, application in the analysis of environmental components; advantages/disadvantages.
13. New trends in instrumental methods with applications in biotechnology.
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| Recommended or Required Reading |
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| Required Reading: |
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CHANG R.: Chemistry. 10th Edition. McGraw-Hill: New York, 2010. 1170 p. Dostupné z: https://archive.org/details/Chemistry_10th_Edition_Raymond_Chang
PERTILE, E. Instrumental Methods of Analysis. Ostrava: Vysoká škola báňská - Technická univerzita Ostrava, 2017. ISBN 978-80-248-4124-3 |
PERTILE E. ČABLÍK V.: Instrumentální metody analýzy, ČSVTS VŠB TUO, 2006, 238 s. ISBN 80-248-1049-2
PERTILE, E., D. SUROVKA: Učební texty k praktiku IMA, multimediální učební text, VŠB TUO 2016
SKOOG, Douglas A., Donald M. WEST, F. James HOLLER a Stanley R. CROUCH. Analytická chemie. Přeložil Karel NESMĚRÁK, přeložil Václav ČERVENÝ, přeložil Tomáš KŘÍŽEK, přeložil Eliška NOVÁKOVÁ. Praha: Vysoká škola chemicko-technologická v Praze, 2019. ISBN 978-80-7592-043-0
PERTILE, E. Instrumental Methods of Analysis. Ostrava: Vysoká škola báňská - Technická univerzita Ostrava, 2017. ISBN 978-80-248-4124-3 |
| Recommended Reading: |
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ROUESSAC Francis and ROUESSAC, Annick: Chemical Analysis Modern Instrumentation Methods and Techniques. March 2007. 600 pp. ISBN: 978-0-470-85903-2
NOLLET M., L., Chromatographic Analysis of the Environment, Chromatographic Science Series, Volume 93, 1297 s. 2006, ISBN 0-8247-2629-4 |
MELOUN M., MILITKÝ J.: Kompendium statistického zpracování dat, Academia Praha 2002 (1. vydání), Academia Praha 2006 (2. vydání), Karolinum Praha 2013 (3. vydání). 985 s. Dostupné z: https://meloun.upce.cz/docs/books/kompendium.pdf
MATĚJKA P. a kol.: Slovník analytické chemie anglicko-český a česko-anglický. VŠCHT Praha. 2005. Dostupné na http://vydavatelstvi.vscht.cz
FLEMR, Vratislav a Eva HOLEČKOVÁ. Úlohy z názvosloví a chemických výpočtů v anorganické chemii. 4. - přeprac. a opr. vyd. Praha: Vysoká škola chemicko-technologická, 2001. ISBN 80-7080-435-1
HARVEY David. Analytical chemistry 2.1. Copyright transferred to David Harvey summer 2016. [online] Dostupné z: http://dpuadweb.depauw.edu/harvey_web/eTextProject/AC2.1Files/AnalChem2.1.pdf |
| Planned learning activities and teaching methods |
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| Lectures, Experimental work in labs, Terrain work, Other activities |
| Assesment methods and criteria |
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| Task Title | Task Type | Maximum Number of Points
(Act. for Subtasks) | Minimum Number of Points for Task Passing |
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| Credit and Examination | Credit and Examination | 100 (100) | 51 |
| Credit | Credit | 33 (33) | 20 |
| Vstupní test | Written test | 4 | 2 |
| Protokoly | Laboratory work | 8 | 5 |
| Písemka | Written test | 5 | 3 |
| Zápočtový test | Written test | 13 | 8 |
| Aktivita | Laboratory work | 3 | 2 |
| Examination | Examination | 67 | 31 |